双酚A对雄性大鼠生殖系统的影响

Effect of bisphenol A on reproductive system of puberty male rats

目的探讨双酚A(BPA)对青春期雄性大鼠生殖系统的影响。方法30只雄性Wistar大鼠,随机分为3组,每组10只。每隔1d腹腔注射低剂量〔1mg/kg·bw〕和高剂量〔25mg/(kg·bw)〕BPA染毒8周,对照组给予等体积溶剂丙二醇。测大鼠体重、睾丸和附睾湿重并计算睾丸/体重比值、附睾/体重比值;观察睾丸、附睾组织学结构的变化;计数附睾尾精子数;应用放射免疫法测定血清性激素水平。结果与对照组比较,低剂量和高剂量BPA组大鼠的体重均下降:高剂量组大鼠死亡2只,睾丸、附睾湿重降低,睾丸/体重比值升高,精子计数减少;光镜下可见睾丸间质水肿,附睾间质血管扩张。结论双酚A可能对青春期雄性大鼠体重、生殖系统的发育及精子发生有不良影响。

Objective To explore the possible effects of bisphenol A(BPA)on the reproductive system of puberty male Wistar rats.Methods Thirty male rats were randomly divided into 3 groups.The successive intraperitoneal administration of BPA at dose of 1mg/(kg·bw) and 25mg/(kg·bw) to rats was conducted in low and high dosage groups for 8 weeks every other day respectively,while rats in control group received the only solvent in equal volume.The body weight,testis and epididymis wet weights,ratios of testis/body weight and epididymis/body weight were determined.The histological observation of the testis and epididymis was carried out and the serum levels of murine sex hormones were detected by radioimmunoassay.Results The murine body weights in both low and high dosage groups were significantly reduced as compared with that of control group.Additionally 2 rats in high dose group died in the process of experiment.Furthermore testis and epididymis wet weights,sperm counts of epididymal obviously reduced,and the ratio of testis/weight increased.Optical microscopy showed the interstitial edema of the testis and the interstitial vascular ectasia of the epididymis.Conclusion BPA might exert probable adverse effects on murine body weight,development of reproductive system and spermatogenesis of puberty male Wistar rats.