摘要
利用农杆菌介导法进行紫花苜蓿遗传转化研究.将从盐生杜氏藻(Dunaliellasalina)中克隆到的抗逆基因Dscbr(编码一种早期光诱导蛋白)导入紫花苜蓿栽培种"中苜一号",获得52个转化株系.经过卡那霉素抗性鉴定和PCR分子检测,获得6株阳性苗,PCR阳性率为11.54%.结果表明Dscbr基因已整合到苜蓿的基因组中.
Alfalfa cultivar ZhongMuYiHao was transformed with Dscbr gene from Dunaliella Salina via Agrobacterium-mediated process. 52 regenerated alfalfa plants were obtained . Kanamycin resistance and PCR amplification analysis showed that 6 putative transgenic plants had the Dscbr gene, and the transformation ratio was 11.54 percent. The stress resistance of putative transgenic plants in laboratory and in field will be tested.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期567-570,共4页
Journal of Sichuan University(Natural Science Edition)
基金
国家高技术863项目(2001AA212161)
