元宝枫组织培养研究

Tissue culture of Acer truncatum

该文对元宝枫茎段组织培养中的外植体灭菌、褐变预防、增殖培养、根诱导等进行了试验研究.结果表明:元宝枫外植体最佳取材时期为4月份,由4年生和2 0年生母树上取材的外植体,经0 1%HgCl2 灭菌6min后,污染率分别降为16 67%和14 2 9% ;对于4龄和2 0龄母树上取材的外植体进行培养时,在培养基中分别添加5 0 0及75 0mg LPVP ,可以较好地控制褐变;当MS培养基中附加0 0 1～0 0 5 μmol LTDZ时,可有效地促进外植体增殖和生长;适宜的生根培养基为1 2MS + 0 1μmol LIBA ,每天光照16h ,生根率可达90 91% ;幼苗经炼苗后盆栽,成活率在95

The stem segments of Acer truncatum were used as explants to study sterilization techniques,browning prevention,proliferation and rooting in vitro . The results show that the optimum season for explant collection is April,the contamination rates of the explants from the 4- and 20-years-old trees are 16.7% and 14.3% after the explants are sterilized in 0.1% aqueous mercuric chloride for 6 min;for the explants from the 4- and 20-years-old trees the addition of PVP of 500 mg/L and 750 mg/L in the medium can prevent browning effectively.The MS medium containing 0.01～ 0.05 μmol/L TDZ is most efficient in the proliferation of Acer truncatum .The ratio of rhizogenesis is 90.91% in the optimum rooting medium of 1/2 MS medium supplemented with 0.1 μmol/L IBA and under 16 h of photoperiod;the survival ratio of transplanting is 95% after training seedling.