乙型肝炎病毒X基因区序列的系统发育树分析

Establishing a new genotyping method for hepatitis B virus by phylogenetic tree construction based on X region sequences

目的:研究利用乙型肝炎病毒X基因区序列构建系统发育树进行基因型分析的可靠性。方法:从美国NCBI基因库中下载HBV全基因序列共2 49条,其中166条为已知基因型的序列,83条为未知型序列。利用ClustalX 1.8软件和TreeView软件对已知基因型的X区序列构建进化树图,分析由该方法获得的基因型是否与原来的吻合,并对未知型序列进行基因型分析,再用S区的进化树分析加以验证。结果:已明确基因型的166条序列利用X基因区序列分析得到的基因型与原先的基因型完全吻合。用X区和S区序列分析方法对83条未知型序列分析的结果是一致的,分别获得A型16条、B型17条、C型2 7条、D型2 1条及F型2条,未发现E、G和H型。结论:利用乙型肝炎病毒X基因区序列进行基因型分析是完全可靠的,有助于HBV的致病机制研究。

Objective To establish a genotyping method for hepatitis B virus (HBV), based on analyzing X region sequences by phylogenetic tree construction. Method Two hundreds and forty-nine complete genomes of HBV were downloaded from NCBI GenBank,of which 166 complete genomes's genotype were known. Phylogenetic grouping of the 166 known sequences on X region was compared with those of original groups. The other unknown 83 complete genomes were analyzed by phylogenetic tree construction and confirmed by S region. Results The outcome of analyzing the 166 clear sequences by constructing phylogenetic tree based on X region proved to be identical with that of original. In 83 unclear genomes the following genotypes were observed:16 A, 17 B, 27 C, 21 D and 2 F. Conclusion This method is reliable and useful for studying the potential influence of genotype on the pathogenesis of HBV.