α-黑素细胞刺激素对体外培养黑素细胞迁移和细胞内钙离子浓度的影响

Effects of α-MSH on melanocyte migration and intracellular [Ca^(2+)]_i in vitro

目的:探讨α黑素细胞刺激素(αMSH)对体外培养黑素细胞迁移和细胞内钙离子浓度([Ca2+]i)的影响。方法:从正常人包皮分离并纯培养黑素细胞,用αMSH处理纯培养的人表皮黑素细胞,用Transwell微孔膜法研究黑素细胞迁移,激光共聚焦扫描显微镜检测[Ca2+]i。结果:αMSH可诱导黑素细胞迁移,并使[Ca2+]i浓度升高。结论:αMSH可能通过诱导黑素细胞内的Ca2+浓度升高,从而促进黑素细胞的迁移。

Objective:To observe the effects of a-melanocyte stimulating hormone(α-MSH) on melanocytes migration and [Ca^(2+)]_i in vitro. Methods:Human melanocytes were obtained from normal foreskins and motility was assessed using the Transwell micropore filter method.Laser confocal microscope was employed to measure [Ca^(2+)]_i. Results:α-MSH could stimulate melanocytes migration through micropore filter and increase [Ca^(2+)]_i . Conclusion:It is suggested that α-MSH might induce melanocytes migration through [Ca^(2+)]_i increasing.