摘要
目的:观察加热对人结肠癌细胞株Lovo的凋亡作用。方法:用Hochest33258荧光染色、凝胶电泳和流式细胞术观察不同时间温度作用下Lovo细胞的变化。结果:不同温度时间处理的细胞均发生凋亡,出现明显的形态学改变及凋亡特有的亚二倍体峰和DNA梯度。其中43℃处理3、4h和44℃处理1、2h时细胞凋亡最明显,细胞周期也发生变化。结论:加热可诱导人结肠癌细胞株Lovo发生凋亡,并具有一定的周期特异性。
Objective:To study the effects of hyperthermia on apoptosis in human colonic cacinoma cell line Lovo. Methods:Lovo cells were exposed to hyperthemia and apoptosis was measured with fluorescent Hocehst 33258 staining, electropherogram gel, flow cytometry (FCM). Results:Significant changes in cell morphology , apoptosis peak apeared before the G1 phase and DNA ladder was observed in various group cells which were exposed to different temperature and in different time. Most significent changes were observed in cells exposed to 43℃ for 3,4 h and 44℃ for 1,2 h. Also cell cycle was found changed. Conclusion:Hyperthermia can cause apoptosis in human colonic cacinoma cell line Lovo, and has phase-specificity.
出处
《医学研究生学报》
CAS
2005年第5期399-401,i015,共4页
Journal of Medical Postgraduates
关键词
加热
结肠癌细胞
凋亡
Hyperthermia
Human colonic cacinoma cell
Apoptosis
