同种异体脱细胞真皮替代同种异体巩膜在眼窝填充中的实验研究

Experimental study of using acelluar dermal dermis instead of donor sclera in orbital implantation

目的①了解同种异体脱细胞真皮植入兔眼窝后的组织相容性。②比较两种不同材料包裹羟基磷灰石(hydroxyapatite,HA)植入的结果,包裹材料及HA的血管化进程。方法随机将24只新西兰大白兔平均分为两组,于球后间隙内分别植入以同种异体巩膜、同种异体脱细胞真皮作为包裹材料的HA,观察大体情况并分别于植入后1、4、8、12周取出,光镜及电镜检查组织学改变。结果大体显示同种异体脱细胞真皮组植入后无一例出现排斥,但其血管化速度较同种异体巩膜组缓慢。结论同种异体脱细胞真皮作为包裹材料有较好的组织相容性,并能逐渐引导被包裹材料的血管化。

Objective To evaluate the histocompatability of homologous acelluar dermal matrix as a wrapping material for a hydroxyapatite orbital implant and compare the speed of fibrovascular ingrowth in hydroxyapatite orbital implant wrapped with homologous acelluar dermal matrix and homologous sclera respectively. Methods Twenty four New Zealand rabbits were divided into two groups randomly .One group is implanted with hydroxyapatite orbital implant wrapped with homologous acelluar dermal matrix behind the globe, the other group with homologous sclera. The observation included immunological reaction of the globe postoperation. Biposies of implanted material were taken on weeks 1, 4, 8 and 12 postoperation for histopathological and electron micrscope examination. Results Histological examination showed the homologous acelluar dermal matrix has no immunological and inflammatory reaction after implanted in orbit. But the speed of fibrovascular ingrowth is a little slow than that of homologous sclera. Conclusions The homologous acelluar dermal matrix has the better histocompatability as an encapsulating biomaterial, it can be a new material instead of homologous sclera.;