几丁糖抑制细菌生长的实验研究

ABSTRACTS$THE STUDY ON BQCTERIOSTATIC ACTION OF CHITOSAN

在表皮葡荡球菌、金黄色扼萄球菌、大肠艾希氏菌、绿脓假单胞菌、白色念珠菌培养液中，按倍比稀释法加入不同剂虽的几丁糖溶液，在３７℃恒温下培养１８小时。观察培养液中细菌生长，得到几丁糖最小抑菌浓度为：表皮葡荡球菌０．００８％，金黄色葡荡球菌０．０１６％，大肠艾希氏菌０．０３２％，绿脓假单胞菌０．０６４％，白色念珠菌１％。实验结果表明，几丁糖溶液具有广谱抗菌作用。

AbstractThe Staphyloccus epidermidis,pseudomonas aeruginosa,Staphylococcus aureus,Escherichi时 acoli,and Candida al bicans were selected as tested micrcorganisms. According to doubling dilutionrule,the chitosan solution of different dosage was added in the culture solution and kept at 37℃constant tcmporature for 18 hours. The smallest bacteriortatic concentration of the chitosan solutionwas 0.016%for Saphylococcus aureus,0.008%for Staphylococcus epidermidis, 0.032%forEscherichia coli,0.064%for pseudomonas aeruginosa, and 1%for Candida albicans, respectively.The experimental result noted that the chitosan solution had a broad-spectrum bacteriostatic action.Key work:Chitossn;Bacterime;Poly-glucosamine