家族性胆固醇结石患者肝组织HMGCR和SCP_2mRNA的表达

Expression of HMGCR, SCP_2 mRNA in hereditary cholesterol gallstone patients

目的:研究家族遗传性胆囊胆固醇结石患者、非家族遗传性胆囊胆固醇结石患者和非胆固醇结石患者肝组织HMGCoA还原酶(HMGCR)、固醇携带蛋白2(SCP2)基因mRNA的表达情况,进而明确二者在胆石形成中的作用.方法:选取2003-08/2004-08天津市南开医院外科住院病例90例,均取得患者及家属知情同意,其中家族遗传性胆囊胆固醇结石患者28例,非家族遗传性胆囊胆固醇结石患者30例,非胆固醇结石患者32例,术中肝活检,行肝组织总RNA提取,逆转录反应合成cDNA,设计引物,进行聚合酶链反应(PCR)扩增HMGCR和SCP2基因的目的片段,检测肝组织mRNA的表达水平的不同.结果:家族遗传性和非家族遗传性胆固醇结石组HMGCR,SCP2mRNA表达水平较非胆固醇结石组升高;其中HMG(:RmRNA表达在家族遗侉}生胆固醇结石组为1.9269±0.2134,非家族遗传性组为1.9791±0.2524,二者均高于非胆固醇结石组0.7730±0.1530,差异有显著性(P<0.05);SCP2mRNA表达在家族遗传性胆固醇结石组为0.8908±0.1649,非家族遗传性组为0.7503±0.1004,二者均高于非胆固醇结石组0.5205±0.1900,差异有显著性(P<0.05);家族遗传性胆固醇结石SCP2mRNA表达水平较非家族遗传性胆固醇结石组升高,差异有显著性(P<0.05),而HMGCRmRNA在家族遗传性和非家族遗传性胆固醇结石组两组间无统计学差别.结论:胆囊胆固醇结石患者肝组织HMGCR,SCP2mRNA表达水平升高是胆囊胆固醇结石形成的重要原因.

AIM: To investigate the expression of the genes for HMGcoA reductase (HMGCR) and sterol carrier protein-2 (SCP2) in the livers of patients with hereditary and non-hereditary cholesterol gallstone, as well as non-cholesterol gallstone. METHODS: The mRNA expression levels of the liver HMGCR and SCP2 genes were determined by RT-PCR in 28 patients with hereditary cholesterol gallstone, 30 patients with non-hereditary cholesterol gallstone, and 32 patients with non-cholesterol gallstone. RESULTS: The mRNA expression levels of HMGCR in hereditary and non-hereditary cholesterol gallstone patients were 1.9 269±0.2 134 and 1.9 791±0.2 524, respectively, both increased significantly (P<0.05) as compared with that in the non-cholesterol gallstone patients (0.7 730±0.1 530). The levels of SCP2mRNA in hereditary and non-hereditary cholesterol gallstone patients were 0.8 908±0.1 649 and 0.7 503±0.1 004, both increased significantly (P<0.05) as compared with non-cholesterol gallstone patients (0.5 205±0.1 900). Moreover, there was also a significant increase of SCP2 mRNA in hereditary cholesterol gallstone patients as compared with non-hereditary cholesterol gallstone patients (P<0.05). CONCLUSION: HMGCR and SCP2 are overexpressed in cholesterol gallstone patients, which may be an important cause of cholesterol gallstones.