大鼠烫伤后肠壁微血管通透性和肠组织含水量的变化

Changes of vascular permeability and water content in ileum post scald in rats

目的:观察大鼠烫伤后12h肠壁微血管通透性和肠组织含水量变化,探讨烫伤早期肠粘膜损伤的可能机制。方法:制作成年大鼠严重烫伤模型(Ⅲ度,30%TBSA),在未烫伤鼠及烫伤后12h应用分光光度法测量肠壁微血管通透性,测量回肠组织含水量。结果:对照组肠壁微血管对伊文思蓝有一定的通透性(12.39±5.062μg/g),烫伤后12h肠壁微血管对伊文思蓝通透性较对照组低(10.50±2.031μg/g),但无统计学意义(t=0.923,P>0.05);对照组回肠含水量为83.3±3.8%,烫伤后12h肠组织含水量比对照组稍低(82.9±1.5%),也无统计学意义(t=0.278,P>0.05)。结论:烫伤早期肠壁微血管通透性和肠组织含水量无显著变化,提示炎症介质在烫伤早期肠粘膜损伤中可能不是主要致病因子。

Objective: To investigate the mechanism of intestinal mucosal injury post scald by observing the changes in vascular permeability and water content in ileum at 12 hours post scald. Method: Adult rats were scalded to form 3rd degree burn wounds with an area of 30%TBSA and their intestinal microvascular permeability and water content in ileum was determined using spectrophotometric method. Result: In control group, intestinal micro - vessels of healthy rats were permeable to Evan Blue (12. 39 ± 5. 062 μg/g). But in experiment group, at 12 hours post scald, microvascular permeability of scalded rats was a little lowered (10. 50 ± 2. 031 μg/g). The difference was not statistically significant (t = 0. 923, P> 0. 05). Water content in ileum was not obviously increased in experiment group and the difference was also not statistically significant (P> 0.05) as compared with the control. Conclusion: The changes in microvascular permeability and water content in ileum at 12 hours post scald were not significant compared with the normal control.