核因子κB激活在大鼠急性胰腺炎发病中的作用

The effect of activated nuclear factor κB in pathogenesis of acute pancreatitis in rats

目的 探讨急性胰腺炎时核因子κB(NF- κB)激活的水平在胰腺炎发病中的作用。方法 将64只Wistar大鼠分成对照组和胰腺炎两组,在制模后分别于12、24、36、72h用流式细胞术(FCM)检测了NF κB激活的水平。同时行病理切片进行胰腺病理分级。结果 急性胰腺炎组中的NF -κB激活的水平在各个时间段均有所增高(P< 0. 05)。在制模后24h胰腺组织中NF κB激活的水平达到高峰,为(62. 17±12 .22)% (P< 0. 01),胰腺组织充血、水肿明显;至36hNF- κB激活趋缓,为(51. 13±11 .72)%,同时胰腺组织出现大片的出血及坏死。结论 在急性胰腺炎的发病过程中,NF- κB的活化作为始动因子激活一系列的炎症介质,引起胰腺组织快速的炎症反应,最终导致胰腺的出血和坏死。

Objective To explore the effect of the activated NF-κB in the pathogenesis of acute pancreatitis in rats.Methods 64 Wistar rats were randomly divided into two groups: pancreatitis group(to be made pancreatitis models)and control group.12,24,36,and 72 hours after the onset of experiment changes of pancrease was observed,pancreatic tissues were stained with HE, and flow cytometry(FCM)were used to examine the activation of NF-κB in the pancreatic tissues. The pathology of pancreatic tissue was graded.Results The levels of activated NF-κB in the pancreatic tissue of pancreatitis group were all signifcantly higher than those of the control group at any time point (all P < 0.05). and Reached its maximum about 24 hours after the onset of experiment that is 62.17±12.22 (all P < 0.01)and then declined at the 36 h time point that is 51.13±11.72. In the pancreatic group severe edema and congestion were found at the 36 h time point and necrosis were found at the 36 hour time point.Conclusion Activated NF-κB as initial factor plays an important role in the pathogenesis of acute pancreatitis, It activates a lot of inflammatory media,and induces cascading reaction of inflammation,and even results bleeding and necrosis of pancreatic tissue.