摘要
将IBD病鸡粪便及饲料经PEG浓缩处理后用RP PCR与单克隆抗体夹心ELISA进行IBDV检测。在RP PCR试验中,粪便和饲料的阳性检出率均为100％(8 8);在夹心ELISA试验中,粪便的阳性检出率为100％(8 8),而在饲料中未检出IBDV(0 8)。试验结果证明,RP PCR是IBDV流行病学特别是传播途径的首选方法。
The feces from chickens with infectious bursal disease (IBD) and feed were treated and concentrated with PEG, and then were used for the detection of IBDV by reverse transcribed polymerase chain reaction (RT-PCR) and sandwich ELISA. The positive rates of both feces and feed were 100%(8/8) in the RT-PCR. In the sandwich ELISA, the positive rate of feces was 100%(8/ 8), but no IBDV was detected in the feed, the positive rate was 0%(0/8). The results indicated that the RT-PCR was a first select method for the detection of IBDV on the study of epidemiology especially for the route of transmission.
