动物组织中四环素类抗生素残留的HPLC检测研究

Study on HPLC for Tetracyclines Residues in Animal Tissues

本试验建立了动物组织中四环素类药物残留的高效液相色谱测定方法。试验分别采用50 mL/L高氯酸、Mcllvaine缓冲液为样品提取液;乙腈/磷酸二氢钠、甲醇乙腈0.01mol/L草酸(1.5+1.5+2.0,pH 2.0)为流动;流速1.0 mL/min,组合测定。结果显示,50 mL/L高氯酸为样品提取液,流动相甲乙腈0.01 mol/L草酸,检测灵敏度较高在此色谱条件下,土霉素、四环素、金霉素的最低检测限为100μg/kg,在100μg/kg^1 000g/kg浓度范围内,标准添加回收率为74.3%~97.1%,变异系数为0.43%~9.54%。本方法适用于四环素类抗生素残留的确证性定量分析。

In order to protect consumer health, determination of the residues in animal tissues by some rapid and accurate methods is a problem demanding prompt solution. A high-pressure liquid chromatography (HPLC) method was performed. The Extraction of analyte was 50 mL/L perchloric acid, chromatographic separations were obtained under a mobile phase of methanol/acetonitrile/oxalic acid (1.5 +1.5 +2.0, pH 2.0) and with a flowrate of 1.0 mL/min. The experiments showed that the limit of quantification was 0.1 μg/g. Good recoveries were observed from 0.1 μg/g to 10 μg/g,with values of 74.3%-97.1%, CV% were 0.43%-11.1%.