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甜菜夜蛾飞行肌中与能量代谢有关的酶活性 被引量:13

Activity of enzymes related to energy metabolism in the flight muscle of beet armyworm
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摘要 通过生化方法测定了参与飞行能源物质代谢的5种酶活力的变化,即3-磷酸甘油醛脱氢酶(GAPDH)、3-磷酸甘油脱氢酶(GDH)、乳酸脱氢酶(L DH)、柠檬酸合酶(CS)和3-羟酰辅酶A脱氢酶(HOAD)。结果表明,1、3、5日龄处女蛾的GAPDH和CS酶活力显著高于7日龄蛾;但HOAD的活性恰恰与之相反,其酶活力随日龄的增加逐渐增强。GDH初羽化时活性较低,但到3日龄后增加到最大值,以后随日龄的增加活性逐渐下降,L DH的活性一直比较低,且不同日龄的酶活力差异不显著。交配状况和幼虫期饥饿处理对成虫的代谢酶活力没有显著影响。在第7代选育家系中,强飞家系的GAPDH酶活力显著高于短飞和不飞;而在第8代中强飞家系的上述5种酶的活性均高于短飞家系,而第10代中仅有CS的活性在强飞和短飞家系中存在显著差异,其他均差异不显著。这说明甜菜夜蛾在初始阶段主要利用糖类作为能源物质,而后期飞行又可通过脂类代谢提供能量,且不同日龄的处女蛾和选择家系的飞行能力与代谢酶活性呈现一定的正相关关系。 In recent years, the beet armyworm, Spodoptera exigua (Hübner), has risen to the status of an economically important pest of many kinds of crops in China, and causes great damage. Based on the research of ourselves and many other research groups, the beet armyworm has been proved to be a strong migrant, but the underlying physiology of its migration is not known. In order to ascertain the energy utilization processes, and understand the physiological mechanisms controlling the onset of migratory behavior, the activities of five key enzymes dealing with energy generation in flight muscle of beet armyworm were measured in the laboratory. They were glyceraldehydephosphate dehydrogenase (GAPDH, glycolytic pathway), glycerol-3-phosphate dehydrogenase (GDH, glycolytic pathway), lactate dehydrogenase (LDH, anaerobic metabolism), citrate synthase (CS, citric acid cycle) and 3-hydroxyacyl-CoA dehydrogenase (HOAD, fatty acid oxidation), respectively. Enzyme activity was estimated with a VERSA max Microplate Reader made by Molecular Devices Corp. USA, with automatic recording of absorption against time, and an assay method according to Beenakkers (1969). Data were analyzed by Soft Max Pro Version 4.3. The results indicated that GAPDH and CS activity levels in 7 day old virgin moths were significantly higher than 1, 3 and 5 day old non-mated adults, while HOAD activities increased steadily with age. GDH activity was very low in newly emerged moths, but increased to maximum levels in 3 day old moths, and then decreased with age thereafter. LDH activity always remained at a very low level and there was no significant difference with age. Mating status and starvation during the larval stage did not affect the activities of the aforementioned enzymes. GAPDH and GDH from the strong-flying line showed significantly higher activity in the 7th and 8th generations, but only CS activities differed significantly between strong-flying and short-flying lines in the 10th generation. Furthermore, there was a positive correlation between flight capacity and the activity of enzymes involved in energy metabolism. These results suggest that beet armyworm will be able to utilize carbohydrate in the initial stages of flight, and then release further energy by the oxidation of lipids.
出处 《生态学报》 CAS CSCD 北大核心 2005年第5期1101-1106,共6页 Acta Ecologica Sinica
基金 国家"十五"攻关课题资助项目 (2 0 0 1BA5 0 BP0 1)~~
关键词 甜菜夜蛾 飞行肌 酶活性 能量代谢 Spodoptera exigua flight muscle enzyme activity energy metabolism
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