白藜芦醇对小鼠氧惊厥的保护作用及其可能机制

Protective effect and possible mechanism of resveratrol on oxygen-induced convulsion in mice

目的:初步探讨白藜芦醇(resveratrol,RV)对小鼠氧惊厥潜伏期及大发作时间的影响及可能机制,以期寻找预防氧惊厥的新途径。方法:应用小鼠氧惊厥模型(纯氧5 ATA暴露),尾静脉注射不同剂量RV(1.5、5、15 mg/kg),采用行为学观察方法,测定氧惊厥潜伏期及大发作时间;并测定RV 5 mg/kg注射剂量下不同时间点(5 ATA氧暴露后5、15、30 min)脑组织丙二醛(maleic dialdehyde, MDA)含量。结果:与对照组相比,RV 5、15 mg/kg组氧惊厥潜伏期及大发作时间均明显延长(P<0.01),而RV 1.5 mg/kg组无显著差异。尾静脉注射RV 5 mg/kg后,RV 5、15、30 min各组顶叶皮质与海马MDA随氧暴露(5 ATA)时间的延长,呈现逐步升高的趋势;但与相应时间点对照组相比,RV 5、15 min组顶叶皮质和海马MDA含量均明显降低(P<0.01),RV 30 min组皮质和海马MDA含量无统计学差异。结论:RV可能通过其抗氧化特性延长氧惊厥潜伏期,从而保护脑组织。

Objective:To explore the effect and possible mechanism of resveratrol(RV) on the latency and duration of convulsion in mice, searching for a new method for preventing oxygen-induced convulsion. Methods: Mice were intravenously injected with RV(1.5 mg/kg, 5 mg/kg, 15 mg/kg body weight),DMSO and NS,and were accordingly divided into 5 groups before exposed to 5 ATA pure oxygen. The latency and duration of convulsion were recorded by observing behavioral changes of mice. The contents of maleic dialdehyde (MDA) in cortex and hippocampus were measured on different time after RV treatment(5 mg/kg body weight). Results: We found that there were significant differences in the latency and duration of oxygen-induced convulsion between the RV group (5 mg/kg and 15 mg/kg) and the control group(P<0.01), whereas there was no significant difference between the RV 1.5 mg/kg group and the control group. MDA contents had an increasing tendency with the progress of exposure, but those in cortex and hippocampus 5 min or 15 min after RV treatment were significantly lower than those in the control groups (P<0.01). Conclusion: RV can prolong the latency of hyperbaric oxygen-induced convulsion through its anti-oxidation effect.