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表达ROG的重组腺相关病毒载体的构建及高滴度病毒的制备 被引量：1

Construction of recombined adeno-associated virus vector harboring mouse repressor of GATA-3 and using it for preparation of high titer virus
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摘要 目的:获得表达小鼠ROG(repressor of GATA 3)基因的重组腺相关病毒载体,制备表达ROG的重组腺相关病毒。方法:设计、合成引物,通过RT PCR法从CTLL 2细胞总RNA中扩增ROG基因,测序确认后,连接于pAAV MCS载体,双酶切、PCR鉴定重组病毒载体;制备高滴度重组腺相关病毒。结果:成功扩增了小鼠ROG基因,构建可表达ROG基因的重组腺相关病毒载体,并获得表达ROG的重组腺相关病毒。结论:本实验获得的小鼠ROG重组腺相关病毒,将为支气管哮喘的基因治疗研究奠定基础。
作者 王桂芳 施柯 杨丹榕 陈吉泉 修清玉 黎怀星 孙树汉
机构地区 第二军医大学长征医院呼吸内科 第二军医大学基础医学部医学遗传学教研室
出处 《第二军医大学学报》 CAS CSCD 北大核心 2005年第4期445-447,共3页 Academic Journal of Second Military Medical University
基金 国家自然科学基金(30170406).
关键词 REPRESSOR of GATA-3 重组腺相关病毒 哮喘 基因治疗
分类号 R562.25 [医药卫生—呼吸系统]
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参考文献8

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二级参考文献11

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第二军医大学学报
2005年 第4期

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