HPLC-荧光法测定中成药中厚朴酚与和厚朴酚含量

Determination of Magnolol and Honokiol in Traditional Chinese Medicinal Preparation by HPLC with Fluorescence

建立了一种用高效液相色谱(HPLC)荧光检测法同时测定中成药中厚朴酚与和厚朴酚含量的新方法.使用BDSC18(100mm×4.0mm,i.d.,3μm)色谱柱,流动相为乙腈0.3%-H3PO4(50∶50,V/V),荧光激发波长为220nm,发射波长为380nm.厚朴酚在0.08～246.0μg/mL范围内线性关系良好,检出限为18.4ng/mL,平均回收率为90.2%￣102.7%,日内RSD为0.80%～4.36%,日间RSD为2.18%～5.92%(n=5);和厚朴酚在0.1～231.0μg/mL范围内线性关系良好,检出限为8.7ng/mL,平均回收率为90.0%～98.1%,日内RSD为0.43%～5.53%,日间RSD为3.97%～6.50%(n=5).该法灵敏度高、选择性好、简便、易重复,为香砂养胃丸、藿香正气软胶囊、国公酒中厚朴酚与和厚朴酚的含量测定提供了有效方法.

A new method for simultaneous determination of magnolol and honokiol in Chinese Traditional Patent Medicine by HPLC with fluorescence detection was established.The sample was separated with a BDS C18 column(100 mm×4.0 mm,i.d.,3μm) and use acetonitrile-0.3%H3PO4(50:50,V/V)as mobile phase.The flow rate was 0.7 ml/min at the column temperature of 45 ℃.The eluate was detected atλem=380 nm with the λex=220 nm.The linear ranges of magnolol and honokiol were from 0.08 to 246 μg/mL(r=0.999 0) and from 0.1 to 231 μg/mL (r=0.999 7) with the detection limits of 18.4 ng/mL and 8.7 ng/mL,respectively.The recoveries(n=5)were 90.2%～102.7% and 90.0%～98.1%. The relative standard deviations of magnolol and honokiol were 0.80%～4.36% and 0.43%～5.53% for within-day,2.18%～5.92% and 3.97%～6.50% for day-to-day.The method was simple,rapid,selective,accurate and has obtained satisfactory results.It can be used for establishing quality stands and control of magnolol and honokiol in Chinese Traditional Patent Medicine.