妥布拉霉素高产菌的选育

Mutation Breeding of High Tobramycin Producing Strain

报道了用紫外线、γ射线、亚硝基胍、吖吖啶橙对妥布拉霉素产生菌──暗黑链霉菌孢子的诱变效应及其原生质体紫外线处理结果。亚硝基胍诱变效果最好，紫外线次之。以亚硝基胍诱变后得到的高产突变株再经紫外线和吖吖啶橙复合处理，获得一高产菌株ＮＵＡ－１３５，其发酵单位是出发菌株的１．６倍，氨甲酰妥布拉霉素单组分的含量由３５％提高到６５．８％，经多次传代证明其遗传性能稳定。

A tobramycin producing strain,S.tenebrarius was treated by UV ,γ-rays NTG ,and acriding orange.It is revealed that the results of NTG treatment are the best.By NTG mutation and combined treatment by UV with acriding orange, a high tobramycin producing strain NUA-153 was screened out.The percentage of carbamoyltobramycin, carbamoyl- kanamycin B, apramycin has changed from 35.0%,30.2%,and 34.8%to 65.8%,5.0%and 26.4% respectively in the fermentation products of this mutant. The proportion of tobramycin is increased 0.8 times as that of the parent strain.The mutant was proved to be stable after successive inoculation culture.