摘要
构建了水貂朊蛋白前体基因的表达载体,所筛选出的阳性克隆质粒经转化表达菌BL21(DE3)后,收获表达菌,纯化鉴定表明获得了目的产物.
A recombinant plasmid designated as pET-32a-MPrP was constructed,which expresses mink PrP precursor fused protein.After transformed the positive recombinant plasmids to BL21(DE3),the expression capacity of the fusion protein were optimized.After ultrasonicated,the sediment was isolated and purified.And the product was confirmed by Western Blot.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第2期180-184,共5页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金资助项目(30270981)
青岛市自然科学基金资助项目
农业部948项目(982070)
农业部疯牛病监测项目.
关键词
水貂朊蛋白前体基因
克隆
序列分析
原核表达
the mink PrP precursor gene
cloning
sequence analysis
prokaryotic expression
