双酚胺酸在小鼠整体放射自显影中的定位分布

Whole-body autoradiographic localization of catecholamic acid in mice

小鼠ⅳ[^(14)C)双酚胺酸370kBq/鼠,不同时间后乙醚麻醉浸入干冰己烷混合液冻死,用整体放射自显影观察^(14)C的分布,5 min,血、肾、胃肠道粘膜、骨、皮毛的放射性最高;肝、肺、脾和唾液腺次之,除骨外20 min至3 h其它组织均降低,24 h,肾和肠还残留少量放射性,但骨中还保持高的放射性。

Catecholamic acid [catechol-3, 6-bis-(methyleneimino diacetic acid)] is a chelating agent synthesized in our institute. Whole-body autoradio-gragphic study was carried out to determine the distribution of [14C]catecholamic acid in 5 adult mice. Each mouse was injected iv 370 kBq. After various times the mice, under ether anesthesia, were immersed in a mixture of dry ice and hexane. Whole-body sections were made at -20 C , 20 μm in thickness. Results showed: (1) At 5 min, higher radioactivities appeared in blood, kidneys, gastrointestinal mucosa, bone, skin, and hair; moderate concentrations in liver, lungs, spleen, and salivary glands. (2) During 20 min and 3 h, the radioactivities decreased (gradually) in all tissues except the bone. (3) Intense radioactivity persisted in the bone, while only traces remained in the kidneys and intestinal mucosa throughout the 24 h period of observation.