摘要
应用离体蟾蜍椎旁神经节(PVG)细胞内记录技术,研究了石杉碱甲(Hup-A)对胆碱能突触传递的影响。Hup-A 0.3或1 μmol·L^(-1)升高节前神经刺激诱发的顺行动作电位发放率并增强外源性ACh电位的幅度,延长时程,但不影响卡巴胆碱电位。结果显示Hup-A是一种高度选择性的ACh-E抑制剂,由此促进PVG的胆碱能传递。
Using the intracellular recording techniques of toad paravertebral ganglia (PVG), the effects of cholinesterase inhibitor huperzine A (Hup-A), an alkaloid first isolated from Huperzia serrata (Thunb) Trev in China, on the synaptic transmission were studied. In 30 PVG cells tested, no remarkable changes in membrane potential and input resistance were observed during superfusion of Hup-A 0. 3 or 1 μmol · L-1 for 15 min. The rate of orthodromic action potential evoked by preganglionic stimulation was increased by Hup-A 0. 3 or 1 μmol·L-1 (n=12, P< 0.05), and much faster, stronger, and longer in action at 50 or 100 μmol · L-1 (n = 11). The amplitude and duration of exogenous acetylcholine-, but not carbachol-, induced depolarization were increased (P<0. 05). It is concluded that Hup-A is a selective and potent cholinesterase inhibitor, by which action it facilitates the cholinergic transmission of PVG neurons.
出处
《中国药理学报》
CSCD
1994年第2期158-161,共4页
Acta Pharmacologica Sinica
基金
Project partially supported by the National Natural Science Foundation of China
the Health Department of Anhui Province.
关键词
石杉碱甲
乙酰胆碱
交感神经节
huperzine A
cholinesterase inhibitors
acetylcholine
carbachol
sympa- thetic ganglia
electrophysiology
