葛根素对猫离体血管平滑肌的作用

Effects of puerarin on cat vascular smooth muscle in vitro

本文研究葛根素对离体血管的作用。异丙肾上腺素使猫的股静脉及肾动脉对甲氧明引发的收缩产生的舒张作用呈剂量依赖式。葛根素能阻滞此种舒张反应,并呈剂量依赖式。普萘洛尔也能阻滞异丙肾上腺素引起的这种舒张。葛根素不能阻滞硝酸甘油引起的舒张反应。这些结果表明葛根素对猫的离体静脉及动脉具有β—受体阻断作用。

Puerarin is an isoflavone compound isolated from Pueraria lobata (Willd). This study characterized the vascular action of puerarin in order to more clearly elucidate its pharmacological activities. Helically cut strips of cat femoral veins and renal arteries were suspended in an organ chamber filled with Krebs bicarbonate solution for measurement of isometric force development. In the cases of femoral veins and renal arteries isoproterenol (0.01 μmol·L-1 -0. 1 mmol·L-1) caused relaxation of methoxamine-induced contraction (0. 01 mmol·L-1) in a concentration -related manner. Puerarin (0.01 - 0. 1 mmol·L-1) inhibited the relaxation response to isoproterenol in a concentration-response fashion. Propra-nolol (0. 1 - 1 μmol·L-1) inhibited the isopro-terenol-induced relaxation too. Puerarin (0. 1 mmol ·L-1) did not alter the relaxation response to nitroglycerin (1 μmol·L-1). These results indicated that puerarin acted as a beta-adrenoreceptor antagonist in isolated arteries and veins.