摘要
用电子自旋共振技术和化学发光法研究了四甲吡嗪(川芎嗪,Lig)对活性氧自由基的清除作用,Lig 25 mg·ml^(-1)对两种水溶液体系产生的O_2和OH的清除率分别为100％和44％。该作用被化学发光法证实。Lig 25mg·ml^(-1)对一种脂溶液体系产生的LOO的清除达80％。提示两性药物Lig能直接清除具细胞毒性作用的自由基(O_2,LOO,OH)。
The scavenging effects of tet-ramethylpyrazine (ligustrazine, Lig) isolated from Ligusticum wallichii Franch on active oxygen free radicals were studied using spin trapping technic and chemiluminescence methods. The scavenging rate of superoxide anion (O2) by Lig (25mg·ml-1) was 100 % in xan-thine/xanthine oxidase (Xan/XO) system, and that of hydroxyl radical (OH) was 44 % in Fenton's reaction. The scavenging rate of lipid peroxyl radical (LOO) by Lig (25 mg ·ml-1) was 80% in peroxidizing microsome system. It is possible that Lig scavenged O2 by catalyzing its spontaneous dismutation. The results showed that Lig had strong effects of scavenging cyotoxic oxygen free radicals (O2, LOO, OH).
出处
《中国药理学报》
CSCD
1994年第3期229-231,共3页
Acta Pharmacologica Sinica
关键词
川芎嗪
化学发光
抗氧化剂
自由基
pyrazines
ligustrazine
electron spin resonance
chemiluminescence
antioxidants
free radicals
