赤子爱胜蚓血小板聚集因子对血小板的活化作用(英文)

Platelet activation by platelet aggregation factor from Eisenia foelide

赤子爱胜蚓血小板聚集剂(EPAF,25.9μmol·L^(-1))能诱导人血小板聚集。EPAF为74.1μmol·L^(-1)时引起5-羟色胺最大释放(89％)。ADP受体拮抗剂(CP/CPK)和阿司匹林均不能抑制EPAF引起的人血小板聚集反应;EPAF(55.6μmol·L^(-1))能诱导凝血酶处理后脱颗粒人血小板产生聚集,表明EPAF诱导。的血小板聚集不依赖于ADP和TXA_2,是一种强血小板激动剂。

A platelet activating factor from earthworm, Eisenia foelide (EPAF, 25-9 μmol·L-1), induced human platelet aggregation and 5-HT (maximal release of 89 % at EPAF 74.1 μmol·L-1) was detected during this process. Neither creatine phosphate/cre-atine phosphate kinase (CP/CPK) nor aspirin completely inhibited the EPAF-induced platelet aggregation. In the presence of fib-rinogen, EPAF (55. 6 μmol·L-1) induced the aggregation of human platelet which had been thrombin-treated and degranulated. Results indicated that EPAF was a potent platelet agonist and the EPAF-induced platelet aggregation was ADP- and TXA2-independent.