西洋参茎叶皂甙单体P－F_（11）对培养大鼠心肌细胞动作电位的作用

Effect of PQS-pseudoginsenoside-F_(11) on action potential of cultivated rat cardiac cells

从西洋参茎叶皂甙中分离提取的Ｐｓｅｕｄｏｇｉｎｓｅｎｏｓｉｄｅ－Ｆ１１（３、１０、３０ｍｇ·Ｌ－１，Ｐ－Ｆ１１）使培养的Ｗｉｓｔａｒ大鼠心肌细胞动作电位的幅值、动作电位时程、阈电位、最大舒张电位、０期最大除极速率和复极１０％、５０％水平的动作电位时程剂量依赖性增大。Ｐ－Ｆ１１（１０ｍｇ·Ｌ－１）的作用可被维拉帕米（２μｍｏｌ·Ｌ－１）所对抗，Ｐ－Ｆ１１（３０ｍｇ·Ｌ－１）与ＢａｙＫ８６４４（０．７５ｍｍｏｌ·Ｌ－１）比较对心肌细胞动作电位的电参数的作用相似。实验结果表明，Ｐ－Ｆ１１可能具有钙通道激活作用。

The Pseudoginsenoside P-F11(P-F11) was extracted from panax quinquefoum saponin(PQS).P-F11(3,10,30 mg·L-1) increased the amplitude of the action potential,overshooting,threshold,the maximum diastolic potential, the maximum rate of depolarization and action potential duration at 10%, 50% levels of repolarization in cultivated wistar rat cardiac cells.These effects were dose-dependent. The effect of the P-F11(10 mg·L-1) was antagonized by verapamil(2μmol·L-1), but was similar to Bay K 8644. The results showed thst P-F11 may be an agonist on calcium channel.