硫氮卓酮对失血性休克犬胰腺的保护作用及其机制的研究

Protective effects of diltizem on pancreas in canine hemorrhagic shock

杂种犬１１只，快速失血使平均动脉压达６．０ｋＰａ．维持９０ｍｉｎ，回输全部血液，２４０ｍｉｎ处死动物。硫氮卓酮（Ｄｉｌｔｉａｙｅｍ，Ｄｉｌ，ｎ＝６）组于休克３０ｍｉｎ时静脉输注Ｄｉｌ（６００１μｇ·ｋｇ－１．１５ｍｉｇ内输完）。休克组在相同时间输入等量生理盐水。结果显示，和休克组比较，Ｄｉｌ治疗能降低胰腺组织黄嘌呤氧化酶（ＸＯ）活性，减少脂质过氧化产物丙二醛生成，增加谷胱甘肽过氧化物酶、Ｃｕ－Ｚｎ超氧化物酶（Ｃｕ－ＺｎＳＯＤ）和Ｍｎ－ＳＯＤ活性。并减轻胰腺损伤程度。提示Ｄｉｌ能保护休克时的胰腺，其机制与减少自由基生成，增加自由基清除酶活性有关。

The study was designed to investigate the protective effect of administration of Diltizem on pancreatic ultrastracture and lipld peroxidation during hemorrhagic Shook in dogs. Anesthetized dogs were rapidly bled to a mean arterial Pressure (MAP) of 6. 0 kPa and maintained at that lever for 90 min, follow by retransfusion with all shed blood.At 30 min after hemorrhagic shock, diltizem (40μg·kg-1·min-1,n=6) and same volume of saline (3ml· kg-1, n=5) were infused intravenously over 15min in diltizem group and in control group,respectivety. At the end of experiment (250 min after hemorrhage and resuscitation)the pancreas samples were obtained and analyzed. The results showed that diltizem treatment significantly ameliorated pancreas ultrastracture damage,exhibited less malonyldialdehyde,lower xathine oxidase(XO)activity and higher activity of Mn-SOD,CuZn SOD and GSH-Px as compared with those of control dogs. These data suggest that diltizem has a beneficial effect on pancreas of dogs with hemorrhagic shock.