3种菊酯类农药对南美白对虾同工酶表型的影响

The effects of three kinds of pyrethrin pesticides on isozymic phenotypes of white-leg shrimp (Penaeus vannamei)

利用聚丙烯酰胺凝胶电泳对注射不同浓度菊酯类农药的南美白对虾Penaeusvannamei3种组织的酯酶同工酶(EST)和苹果酸脱氢酶同工酶(MDH)进行了分析比较。结果表明:经农药胁迫后,虾体内各组织同工酶酶带出现明显的变化,在不同浓度下存在明显的差异,并具有明显的组织特异性。由于不同种农药的作用结合位点不同,使得酶带变化规律也不相同,当虾体被注射农药后,EST酶带变化较复杂,而MDH酶带则较稳定。当虾体受到农药胁迫时,可引起虾体无氧代谢加强,有氧代谢和酯类降解代谢减弱,正常的物质代谢平衡被破坏,因而使供给机体的能量减少,机体的免疫力和抵抗外界环境的能力均明显降低;另外,还有可能诱发虾病的暴发或导致虾的大批量死亡。

Esterase isoenzyme (EST) and malate dehydrogenase isoenzyme (MDH) were analyzed in three tissues (muscle, hepatopancreas and intestine)in white-leg shrimp (Penaeus vannamei) injected with different concentrations of pyrethrin pesticide by polyacrylamide gel electrophoresis (PAGE). The results showed that the significant variations were observed in the isozyme bands of the shrimp injected with the pesticide at various dosages, and specific distinctions in the tissues. There was complicated change in the EST bands and stable in the MDH because the bonding point of the pesticides were different. The shrimp stressed by pesticides had high anaerobic metabolism and low aerobic metabolism, which results in decline in resistance to exotic environment and in the immunity.