摘要
体外观察CdCl2对大鼠肝线粒体和微粒体膜流动性,膜结合酶活性,脂质过氧化产物丙二醛(MDA)生成的影响以及维生素C的拮抗作用。结果表明,Cd2+可在较短时间内引起膜流动性的降低和MDA的增高,并伴有膜结合酶活性的抑制;维生素C处理不能改善膜流动性的降低。但能完全拮抗MDA的增高,并部分缓解Cd2+对葡萄糖-6-磷酸酶的抑制,提示Cd2+膜毒性并非单纯由脂质过氧化所致,其中包括了对膜脂双层的直接作用。
The effects of Cd2+ on rat hepaticmitochondrial and microsomal membrane fluidities.membrane-bound enzyme acdvides.malondidldehyde(MDA)and vitamin C antagonistic action were investi-gated.Tberesults show that the fluorescencepolarization of 1,6-diphenyl-1,3,5-hexatriene labeledon the membranes ofmitochondha and microsomes issignificantly increased. The activities of Ca2+ ATPase,G-6-PaseNADPH-cvtOchrome Cfcductase aremarkedly inhibited by Cd2+ in the range of 1 umol·L-1 to 10 mmol·L-1 .The increase of MDA inmitochondria and microsomes due to Cd2+ was alsoobserved at the final concentration of 25 mmol·L-1 and 25 umol·L-1The increased M DA and de-creased fluiditv of the membranes had a good correla-tion and they all happened in a very short time. Vita-minctreatment had no effect on the fluidity ofmicrosomal membranes, but completely antagonisedthe increase of MDA and partly reduced the degree ofG-6-pase inhibition. The possible mechanism in-volved waS discussed.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1994年第1期60-64,共5页
Chinese Journal of Pharmacology and Toxicology
关键词
氯化镉
维生素C
膜
膜流动性
cadmium chlorde
vitamin C:membrane:membrane fluidity
membrane-boundenzymes
lipid peroxidation