兴奋性氨基酸受体拮抗剂对大鼠缺血海马磷脂酰肌醇代谢的影响

Effects of excitatory amino acid receptor antagonists on Phospha-tidylinositol tornover in rat hippocampus under cerebral ischemia

兴奋性氨基酸受体拮抗剂对大鼠缺血海马磷脂酰肌醇代谢的影响卢步峰，鲁友明，黄森（镇江医学院生物化学研究室，镇江２１２００１；海军神经生物学研究中心分子药理学研究室，南京２１００４９）代谢型兴奋性氨基酸受体偶联的第二信使肌醇１，４，５一三磷酸（ｉｎｏｓｉ...

An increment of phosphatidyl- inositol(PI) hydrolysis was observed as directly studied inhippocampus of the adult Sprague-Dawley ratreceiving 10 min of cerebral ischemia. 2-Amino-3phosphonopropionic acid (16.9 ng, by intrahippo-campalinjection 5 min before isehemia) or daurisoline (1 mg· kg-1 ip 20 min before ischemia) markedlydecreased the activation of PI tumover (P<0.01).Ketamine(100 mg · kg-1, ip) and mmodipine (0.5 mg·kg-1, ip) only brought about a slight reduction in theischemia-induced PI hydrolysis. These results suggest thatthe activation of PI turnover in this isehemic model is ametabotropic excitatory anuno acid naptor-mediated re-sponse, which supports the idea that glutamaterelease indutal by cerebral isehenua plays a role inneurotoxiclty.