牛膝多糖对T淋巴细胞和天然杀伤细胞功能的影响

Innuence of Achyranthes bidentata polysaccharides on function of T lymphocytes and natural killer cells

牛膝多糖（ＡＢＰ）是从中药牛膝根中分离得到的一种有效成分。ＡＢＰ５０－８００ｍｇ·Ｌ－１在体外增强天然杀伤（ＮＫ）细胞活性和促进伴刀豆球蛋白Ａ（ＣｏｎＡ）诱导的肿瘤坏死因子－β（ＴＮＦ－β）产生；但不能提高ＣｏｎＡ诱导的Ｔ淋已细胞增殖反应和白介素２的产生．ＡＢＰ５０及１００ｍｇ·ｋｇ－１ｉｐ明显提高正常小鼠ＮＫ细胞活性和ＴＮＦ─β生成，增强二硝基氟苯诱导的迟发型超敏反应和对抗环磷酰胺对ＮＫ活性的抑制作用。但对ＣｏｎＡ诱导的Ｔ淋巴细胞增殖反应和白介素２的产生无明显影响。表明ＡＢＰ对Ｔ淋巴细胞功能的影响是有选择性的．ＡＢＰ对ＮＫ细胞的杀伤活性的增强作用是明显的．

Achyranthes bidentatapolysaccharides(ABP),50-800 mg·L-1 couldpotentiate natural killer(NK)cell activity againstYAC-1 cells and increase the Con A(5 mg·L-1)-induced production of tumor necrosis factor β(TNF-β)from murine splenocytes.However,ABP didnot show any significant effect on both the Con A-in-duced splenocyte proliferation and IL-2 production invitro.ABP 50,100 mg·kg-1 ip markedly promotedNK cell activity of normal mice as well as im-munodepressed mice resulted from cyclophosphamidetreatment.In addition,ABP also raised TNF β pro-duction from murine splenocytes and enhanced the de-layed type hypersensitivity reaction caused by dinitro-fluorobenzene:but ABP had no obvious influence onCon A-induced splenocyte proliferation and IL-2production in vivo. Above results indicated that someof the T lymphocyte-mediated immune functions werepotentiated by ABP and that ABP possessed an evi-dent enhancement on NK cell activity.