灵芝多糖引起的小鼠脾细胞核DNA，RNA含量及核质比的变化

Changes of nuclear DNA,KNA contents and ratio of nucleus to cytoplasm of murine splenocytes induced by Ganoderma lucidum polysaccharides

灵芝多糖可引起体外培养的小鼠脾细胞核ＤＮＡ，ＲＮＡ含量显著增加，细胞内超微结构改变，细胞质和细胞核的平均截面积明显增加，核质比呈下降趋势，提示灵芝多糖诱导脾细胞ＤＮＡ和蛋白质合成的同时，促进细胞增殖。

The changcs of nuclear DNA,RNAcontents and ratio of nucleus to cytoplasm of murine spleen cells mediated by Ganoderma lucidum polysaccharides(GLP)were studied using acytofluorophotometer and electron microscope.It wasfound that GLP 50-200 mg·L-1 not only increasedthe contents of nuclear DNA and RNA but also re-markably changed the cell ultrastructure and meancross section areas of nucleus and cytoplasm and re-duced the ratio of nucleus to cytoplasm.These resultsindicated that GLP could increase the synthesis of nu-clear acid and protein and promote cell proliferation inmurine splenocvtes.