雌二醇诱发中国仓鼠肺细胞有丝分裂纺锤体损伤

Mitotic spindle damage induced by estradiol inChinese hamster fibroblast cells

本文用同时显示纺锤体和染色体的分化染色技术，研究了雌二醇（Ｅｓｔ）对中国仓鼠肺细胞纺锤体的损伤作用．实验结果表明，随着培养液中Ｅｓｔ浓度的寸高。纺锤体形态正常的分裂相明显减少，在本实验的最高浓度（５０．０ｍｇ·Ｌ－１）已降为０，说明Ｅｓｔ是一种纺锤体毒物．纺锤体异常的分裂相有２个主要类型，一是非中板集合，二是无极分裂相．当Ｅｓｔ浓度小于５．０ｍｇ·Ｌ－１时．纺锤体异常以非中板集合为主，最高值为４７．５％．其它类型的纺锤体异常如不分离．滞后，单极和多极分裂相等，其剂量效应关系无规律性变化．

By using differential staining tech-nique for simultaneous visualization of mitotic spindleand chromosomes, this paper studied the mitotic spin-dle damage induced by estradiol in Chinese hamsterfibroblast cell line (CHL) cells. Experimental resultsshow that following the increase of estradiol concentra-tions the number of metaphases with nonnalmorphology of spindles decrease obviously. And thereis no nonnal spindle can be seen at the highest concen-tration (50.0 mg . L-1). That means estradiol is a sortof spindle poison. There are two kinds of abnormalspindle metaphases, one is non-congression and theother is apolar. When the concentration of estradiol isless than 5.0 mg . L-1 the main kind of abnonnal spin-le is non-congression and its highest value is 47.5%.The dose-effect relationships of other kinds of abnor-mal spindles such as non-disjunction, lagging,monopolar and multipolar do not show regularchanges. The similarities and the differences betweenestradiol and colchicine as well-known spindle poisonregarding the induction of spindle damage are brieflydiscussed.