胡芦巴中皂苷成分的研究Ⅲ.新皂苷D的分离纯化及化学结构测定

Studies on saponins from seeds of Trigonella foenum-graecum Ⅲ. Isolation and structural elucidation for a new saponin D

目的对胡芦巴Trigonellafoenum-graecum种子所含的皂苷成分做深入研究。方法采用硅胶H柱色谱、干柱色谱等从总皂苷中分离纯化单一皂苷成分,并应用13C-NMR、FAB-MS、DEPT等光谱及部分水解获得的次生苷的方法测定其化学结构。结果从总皂苷中分离出的一个新皂苷D为六分子糖与薯蓣皂苷元构成的六糖苷,经测定皂苷D的结构为:薯蓣皂苷元-3-O-α-L-鼠李吡喃糖(1→3)-β-D-葡萄吡喃糖(1→4)-α-L-鼠李吡喃糖[(1→3)-α-L-鼠李吡喃糖](1→4)-β-D-葡萄吡喃糖(1→4)-β-D-葡萄吡喃糖苷。结论皂苷D是一个新的六糖皂苷。

Objective To investigate the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum-graecum L.). Methods To isolate and purify the single saponin by the column chromatography and dry column chromatography of silica gel H. The structural elucidation was carried on by spectroscopic evidence of (()^(13)C-NMR), FAB-MS, DEPT and the results of the fraction-hydrolysis of acquiring the secondary glucosides. Results The newly isolated saponin D was consisted by six molecules of sugar with diosgenin. The chemical structure of saponin D is: diosgenin-3-O-α-L-rhamnopyranosyl (1→3)-β-D-glucopyranosyl (1→4)-α-L-rhamnopyranosyl [(1→3)-α-L-rhamnopyranosyl] (1→4)-β-D-glucopyranosyl (1→4)-β-D-glucopyranoside. Conclusion Saponin D is a new one consisted of six molecules of su-(gar) with diosgenin.