过氧化氢酶过度表达对血管平滑肌细胞增殖的影响

Effect of overexpression of catalase on proliferation of vascular smooth muscle cells

目的:探讨腺病毒载体介导的过氧化氢酶基因转染对体外培养的人血管平滑肌细胞增殖的影响。方法:用含过氧化氢酶基因的重组腺病毒(AdCat)转染人血管平滑肌细胞,应用四甲基偶氮唑蓝(MTT)方法观察血管平滑肌细胞的增殖活性,同时用流式细胞术观察血管平滑肌细胞细胞周期的变化。结果:MTT比色法分析显示AdCat组明显抑制细胞增殖,与对照组相比差异有显著性(P<0.05);细胞周期分析发现AdCat组G0/G1期分布百分率显著高于对照组,而S、G2/M期分布百分率显著低于对照组,细胞增殖能力下降(P<0.05)。结论:腺病毒载体介导的过氧化氢酶基因转染能抑制人血管平滑肌细胞的增殖。

Objective To investigate the effect of infection with adenoviral vector containing cDNA for human catalase on proliferation of cultured human vascular smooth muscle cells(VSMC). Methods Human VSMC were infected with catalase- contained recombinant adenovirus(AdCat) in vitro. The VSMC proliferation was determined by MTT test. Cell cycle was observed by flow cytometry. Results AdCat restrained the proliferation of VSMC apparently with the peak of cell growth being attenuated. Analysis of cell cycle indicated that the ability of cell proliferation decreased significantly in VSMC infected with AdCat as compared with the control group ( P< 0.05) . Conclusion These findings indicate that infection with AdCat inhibited proliferation in VSMC. [