非促有丝分裂型人酸性成纤维细胞生长因子对细胞增殖活性的影响

The mitogenic activity of the non-mitogenetic human acidic fibroblast growth factor on nasopharyngeal and angioendothelial cells

目的研究观察人酸性成纤维细胞生长因子(haFGF)和N端缺失27个氨基酸残基的非促分裂人酸性成纤维细胞生长因子(non-mitogenetichumanacidicfibroblastgrowthfactor,nm-haFGF)对鼻咽癌细胞及血管内皮细胞增殖活性的影响。方法用不同浓度(0.01～100ng/ml)的人酸性成纤维细胞生长因子(haFGF)及nm-haFGF分别刺激鼻咽癌细胞及血管内皮细胞,作用48h后采用MTT法测定haFGF和nm-haFGF对两种细胞的增殖活性。结果在各浓度(>0.01ng/ml)下,nm-haFGF组对鼻咽癌细胞(除浓度为50ng/ml外)和血管内皮细胞的促增殖作用都显著低于haFGF组。结论nm-haFGF对鼻咽癌细胞及血管内皮细胞的促分裂活性下降。

Objective To study the mitogenesis of non-mitogenetic human acidic fibroblast growth factor (nm-haFGF) on nasopharyngeal and angioendothelial cells. Methods The mitogenic effects of human acidic fibroblast growth factor (haFGF) and nm-haFGF on CNE2 cells and ECV304 cells were measured by MTT method. Results At different concentration(>0.01 (ng/ml)), the mitogenic effects of nm-haFGF on nasopharyngeal cells(except for the concentration of 50 (ng/ml)) and angioendothelial cells was significantly lower than that of haFGF. Conclusion The mitogenic effect of nm-haFGF on CNE2 cells and ECV304cells is lower than that of haFGF.