半边旗5F对瘢痕成纤维细胞增殖和凋亡的影响

Effect of 5F from Pteris semipinnata L. on proliferation and apoptosis of scar fibroblasts

目的通过对天然药物半边旗5F对瘢痕成纤维细胞凋亡作用的研究,以期探寻新的治疗病理性瘢痕的有效药物。方法以四甲基偶氮唑(MTT)法、流式细胞术和原位末端标记技术检测半边旗5F对成纤维细胞增殖和凋亡的影响。结果半边旗5F对成纤维细胞的增殖具有显著的抑制作用,且呈现浓度和时间依赖性。采用流式细胞术和TUNEL法检测凋亡指数,均呈剂量依赖性增高(P<0.05)。结论半边旗5F对瘢痕成纤维细胞的增殖具有显著的抑制作用,并且能够诱导其发生凋亡,为寻找有效治疗增生性瘢痕的药物进行了新的尝试。

Objective To study the effect of herbal medicine Pteris semipinnata L on proliferation and apoptosis of scar-derived fibroblasts and search for drugs for human pathological scar. Methods Fibroblasts were cultured as an experimental model and the effect of Pteris semipinnata L was examined. Cell proliferation was determined by MTT assay, and apoptosis of fibroblasts induced by Pteris semipinnata L was analyzed by flow cytometry and TUNEL. Results Pteris semipinnata L significantly inhibited the proliferation of fibroblasts, and elicited apoptosis of cultured fibroblasts(P<0.05). Conclusion Pteris semipinnata L can significantly inhibit proliferation and induce apoptosis of cultured fibroblasts, suggesting its potential role as a new drug for the treatment of pathological scar.