射频消融处理时间对软骨基质影响的初步观察

Preliminary Obervation on the Effects of Radiofrequency Energy Treatment's Time on Matrix of Fibrillated Articular Cartilage

建立膝关节软骨退变模型,模拟膝关节镜环境,观察射频消融能量在不同处理时间对关节软骨的即时损伤。射频消融气化仪在同一能量设置条件下处理牛膝关节退变软骨,按处理时间分为对照组、10s组、30s组、60s组,共4组,每组6份标本,随后行软骨组织块培养,阿利新蓝法测定组织块每日GAG释放率以确定基质的损伤程度,SEM观察软骨网状胶原纤维的超微结构改变。随着射频消融处理时间的增加,GAG释放率明显减少,培养后第3天,各组释放率较第1天都明显减少,30s组、60s组GAG释放率几乎为0。SEM超微结构提示,网状胶原纤维出现排列紊乱,正常网状结构消失,结构致密,胶原纤维断裂。随着射频消融处理时间的延长,软骨基质损伤明显加重,射频消融处理时间与关节软骨基质的损伤呈正相关。

The paper sets up the partten of the mechanically traumatized cartilage,in a setting designed to mimic an arthroscopic procedure,to determine the effects of radiofrequency energy treatment's time matrix of fibrillated articular cartilage.4 groups with 24 samples were obtained from 3 cattle,sknees,one group served as the control while the samples was treated in the same setting for 10sec s,30sec s,60sec s.Cartilage explants were incubated with culture medium,matrix damages were assessed by inspection of the microstructure of the collagen fiber network through SEM and by measuring the release of glycosaminoglycans to the culture media using the DMB assay.With the increase of radiofrequency energy treatment,s time,GAG release was significantly decreased,compare to the first day, GAG release was significantly decreased in the third day.In the third day,GAG release of the group of 30sec s and 60sec s was almost zero.SEM:the collagen fiber network was significantly damaged,the normal structure of network was vanished and fiber was breaken.With the increase of radiofrequency energy treatment,s time,damages of the matrix of fibrillated articular cartilage was significantly servere.The damages of the matrix and the time of the radiofrequency energy treatment were positive correlation.