摘要
纯培养技术一直是微生物学研究的基石,但其单一的营养结构和生境与自然环境中微生物多样性、协同代谢等明显矛盾,从而成为部分微生物难以复苏的主要障碍。细菌共同协作的自然生存方式的崩溃、生境的极度营养变化和生态位巨变等是微生物可培养性低的主要生态学原因。非培养技术、加富培养、混合培养、稀释培养、模拟自然培养和综合方法等是主要的研究手段和策略。
Pure culture technique has been a fundamental method since its invention in microbiology, but its isolated and monotonous environment contradicts microbial diversity and cooperation relationship in natural environment. And this contradiction directly results in the uncultivability of some microbes in media. From ecological viewpoint, the recovery barriers of natural microbes mainly include the crash of cooperation in natural environment, the great change of nutrient in new environment and the loss of native niche. The main methods and strategies on low curturability of microorganisms are culture-independent methods, enrichment culture, mixed culture, dilution culture, simulating nature culture and synthetic method, which can to some degree compensate for the traditional approach and improve the low culturability of some bacteria.
出处
《微生物学报》
CAS
CSCD
北大核心
2005年第3期478-482,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金 ( 5 0 3 780 95 )
重庆大学骨干教师基金( 7164 110 45 )~~
关键词
微生物多样性
可培养性
培养技术
未培养微生物
Microbial diversity, Culturability, Culture technique, Nonculturable microorganism