用反相HPLC－电化学法研究人体内复方多巴片的生物利用度

Bioavailability study of levodopa and carbidopa in ten healthy male vol-unteers by reversed-phase HPLC-ED

用反相高效液相色谱－电化学（ＨＰＬＣ－ＥＤ）法对复方多巴进口片、国产甲片和国产乙片进行人体生物利用度研究，１０名健康志愿者采用交叉、平行、对照法口服３种复方多巴片后，测定１４ｈ内不同时间的血药浓度。结果表明，国产片与进口片的相对生物利用度：左旋多巴国产甲片为９９．４０％，乙片为１０４．３０％；卡比多巴国产甲片为９９．９２％，乙片为１０２．７９％，国产片与进口片的药动学参数除左旋多巴中甲片的Ｔ＿（ｍａｘ）、乙片的Ｃ＿（ｍａｘ）、Ｔ＿（１／２）有非常显著性差异（Ｐ＜０．０１）外，其它各项丰数均无显主性差异（Ｐ＞０．０５）。

The bioavailability of levodopa-carbidopa tablets was investigated in ten healthyvoluteers after oral administration. Three kinds of levodopa-carbidopa tablets including sinemet(USA)and A Chinesetablet and B Chinese tablet were used to compare the bioavailability in bodywith cross ,parallel,contrast.The plasma of levodopa-carbidopa levels for 14 hours were deter-mined by HPLC-ED.Pharmacokinetic parameters were fitted with PKBP-N1 program on comput-er. The results show that the comparative bioavailability of A Chinese tablet was 99.40% of lev-odopa and 99. 92% of carbidopa,and that of B Chinese tablet was 104.30% of levodopa and102.79% of carbidopa.Excepting T_(max) of levodopa in A Chinese and C_(max)、T_(1/2) of levodopa in BChinese were significantly different(P<0.01)between foreign and Chinese tablet,there was nosignificant difference (P> 0.05)between foreign and Chinese tablet on other pharmacokintic pa-rameters.