超氧化物歧化酶测活方法的比较

Comparison of assay methods of SOD

超氧化物歧化酶测活方法很多，国外以黄嘌呤氧化酶－细胞色素Ｃ法和经典的连苯三酚自氧化法为主，国内以改良的连苯三酚自氧化法和黄嘌呤氧化酶－ＮＢＴ法为主，测活方法不一，所测活性单位也不一，测试结果很难比较。为便于与国际上公认的ＳＯＤ测活方法比较，找出同一酶制剂在各种活性测定中的相互关系，这将有助于改变我国当前活性测定的紊乱现象。本文内容主要对国内外最重要的３种测活方法进行了比较。

There are many assay methods for superoxide dismutase(SOD),The assay methodsare main xanthinoxidation-Cytochrome C(550nm method)and pyrogallol autoxidation(420nmmethod)in foreign country,and pyrogallol autoxdation (325nm method)in country.But it is dif-ferent to compare the data obtained by these methods. We compared four methods for SODactivity.