气相色谱法测定降香挥发油中反式苦橙油醇的含量

Determination of nerolidol in volatile oil of Dalbergia odorifera by GC

目的:建立降香挥发油中反式苦橙油醇的气相色谱含量测定方法。方法:采用AgilentHP-5柱(320μm×30m,0.25μm);流动相高纯N2,流速2mL·min-1。结果:反式苦橙油醇在0.059-1.97mg·mL-1呈良好线性关系(r=0.9997),平均回收率97.5%,重复性RSD2.3%。结论:本法简便,准确且重复性好,可有效控制该药材挥发油中反式苦橙油醇的含量。

Objective: To develop a method for determination of nerolidol in the volatile oil of Dalbergia odorifera. Method: GC method was used. The samples were separated on Agilent HP-5 column (320μm × 30 m, 0.25μm) with the mobile phase of highly pure N2. How rate was 2 mL-min-1. Result: Linearity of nerolidol was good linearity in the range of 0.059-1.97 mg'mL-1, and the average recovery was 97.5% , RSD 2.3%. Conclusion: This method is simple, accurate, rapid and reproducible.