四物汤对综合放血法致小鼠血虚证造血功能的影响

Hematopoietic effect of Siwu decoction in the mice with blood deficiency induced by compound method of bleeding, starved feeding and exhausting

目的:观察四物汤对综合放血法致小鼠血虚证造血功能的影响,为比较四物汤对不同血虚证模型补血作用提供实验依据。方法:每日按75g·kg-1体重限食,隔日游泳20min及放血0.4mL共15d,建立小鼠血虚证模型。提前7d,四物汤小,中,大剂量组分别按每天5,10,20g·kg-1给予四物汤至造模结束,通过血细胞计数仪及流式细胞仪等分析手段,检测外周血象、骨髓细胞周期、骨髓细胞凋亡和CD34+细胞数。结果:四物汤小,中,大剂量组可以使血虚证小鼠红细胞由第7天最低值4.6×1012·L-1,分别显著升高到6.5,7.1,7.0×1012·L-1;在第15天,使血虚证小鼠S期骨髓细胞数由11.53%,分别显著增加到14.34%,14.03%,13.58%。结论:四物汤可以在一定程度上逆转综合放血致小鼠的造血损伤。

Objective: To observe the hematopoietic effects of Siwu decoction in the mice with blood deficiency induced by compound method of bleeding, starved feeding and exhausting and lay experimental basis under the blood-enriching function of Siwu decoction in different models of blood deficiency. Method: The animal model of blood deficiency was established by a combination of starved feeding with routine food at the rate of 75 g·kg-1 body weight per day, swimming 20 minutes and removing 0.4 mL blood every other day in each mouse. The amount of peripheral blood cells, the cell cycle and the apoptosis of bone marrow cells and the percentage of CD34+ in bone marrow cells were detected by microcellcounter and flow cytometry. Result: The amount of red blood cells in model mice was significantly increased by Siwu decoction at different dosages, from 4.6 ×1012to 6.5, 7.1 and 7.0 ×1012respectively. At the 15th day, the percentages of bone marrow cells at S stage in the treated group were 14.34% , 14.03% and 13.58% which were significantly higher than that in model group without treatment of Siwu Decoction. Conclusion: Siwu decoction significantly improve the hematopoiesis in the mice with blood deficiency induced by compound method mentioned above.