左旋四氢巴马汀对脂多糖诱导人脐静脉内皮细胞黏附分子表达的影响

Effect of l-tetrahydropalmatine on expression of adhesion molecules induced by lipopolysaccharides in human umbilical vein endothelium cell

目的:观察左旋四氢巴马汀(l-THP)对脂多糖(lipopolysaccharides,LPS)诱导人脐静脉内皮细胞(HU-VEC)细胞间黏附分子-1(ICAM-1)和E选择素(E-selectin)表达的影响,探讨l-THP的抗炎作用。方法:分离纯化人脐静脉内皮细胞,与LPS或LPS加不同浓度l-THP共育,用流式细胞仪检测细胞表面黏附分子表达的变化。结果:l-THP(250mg·L-1)显著降低LPS诱导的ICAM-1和E-selectin表达(P<0.05)。l-THP(50mg·L-1)抑制E-selectin表达(P<0.05),然而对ICAM-1的表达无明显影响(P>0.05)。l-THP(10mg·L-1)对2种黏附分子表达均无明显影响(P>0.05)。50mg·L-1地塞米松(DXM)完全抑制LPS诱导上述2种黏附分子的表达(P<0.05)。结论:左旋四氢巴马汀能减少LPS诱导的ICAM-1和E-selectin表达,提示l-THP可开发成炎症治疗药物。

Objective: To investigate the effect of l-tetrahydropalmatine (l-THP) on adhesion molecule expression induced by LPS in human umbilical vein endothelium cell (HUVEC). Method: HUVEC were obtained from the human umbilical cord vein and treated by LPS or LPS plus different concentration of l-THP. Expression of ICAM-1 and E-selectin was assayed by flow cytometer. Result: l-THP(250 mg·L-1)markedly attenuated ICAM-1 and E-selectin expression induced by LPS(P<0.05) . l-THP(50 mg·L-1) inhibited E-selectin expression (P<0.05) but had no effect on ICAM-1(P> 0.05). l-THP(10 mg·L-1) had no effect on expression of both adhesion molecules. DXM(50 mg·L-1) completely inhibited both responses induced by LPS (P<0.05). Conclusion: l-THP depresses expression of ICAM-1 and E-selectin induced by LPS, which suggests that l-THP represent a promising candidate to be developed into therapies for the treatment of inflammation.