rIL_（－2）对慢性HBV感染者NK细胞功能调节及影响因素

THE EFFECT OF rIL- 2 ON THE REGULATION OF NK CELL FUNCTIONS AND ITS INFLUENTIAL FACTORS IN CHRONIC HBV INFECTIONS

本文采用４小时、１６小时微量释放细胞毒法监测慢性ＨＢＶ感染者ＮＫ细胞对Ｋ５６２细胞及ＰＬＣ／ＰＲＦ／５细胞杀伤活性及某些影响因素。实验提示慢性活动型肝炎（ＣＡＨ）、慢性无症状携带者（ＡＳＣ）ＮＫ细胞杀伤活性均高于健康人。ｒＩＬ－２可明显增加健康人ＮＫ细胞活性，但对ＨＢＶ感染者ＮＫ细胞增强作用不明显。ＨＢｓＡｇ、ｒＨＢｅＡｇ和ｒＨＢｃＡｇ均可明显抑制ＮＫ细胞活性，并有剂量依赖性，加入外源性ｒＩＬ－２仍不能纠正。提示ＨＢｓＡｇ、ｒＨＢｅＡｇ和ｒＨＢｃＡｇ可通过某种机制阻抑ｒＩＬ－２与ＮＫ细胞结合发挥作用。

The natural killer(NK) cell activity and its influential factors o fchronic HBV infected Peripheral blood lymphocytes were studid by 51 Cr releasing cytotoxicity assay against k562 and PLC/PRF/5 target cells.The NK cell activity was found to be higher in chronic HBV infections than that in normal control group. By the addition of rIL-2 in vitro, normal control NK cell cytosinic acitvity was higher than absence of rIL-2,but this was not found in patient group.The influence of Purified HBsAg, rHBeAg and rHBcAG inhuman NK activity was also examined. Lymphocytes preincubated with HBsAg and rHBcAg and subsequently showed a significant decrease in NK cytotosity against K 562 target cells.The effect was dose-dependent and rIL-2 could not be corret.These results suggested that HBV or its products might inhibibt rIL-2 effect on NK cell in some way.