甲型肝炎早期诊断──酶联捕获法检测唾液IgM－抗HAV研究

A STUDY OF EARLY DIAGNOSIS OF HEPATITIS A-ELISA FOR DETECTION OF ANTI-HAV-IgM IN SALIVA

对７０例急性肝炎患者，用酶联捕获法检测血清和唾液ＩｇＭ－抗ＨＡＶ，证实为甲型肝炎者５４例，包括甲、乙型肝炎混合感染１９例。５４例患者血清与唾液ＩｇＭ－抗ＨＡＶ阳性符合率９２．６％（５０／５４）。在８１例对照组中，血清与唾液均未测出ＩｇＭ－抗ＨＡＶ，两者阳性及阴性总符合率９７．４％。（１４７／１５１）。唾液抗体滴度１０－１至１０－３比血清抗体滴度１０－３至１０－６为低。但低滴度并不影响酶联反应的结果，本组资料表明病人唾液ＩｇＭ－抗ＨＡＶ阳性可做为甲型肝炎早期诊断的依据，唾液标本具有采集容易，操作简便，检测结是容易判定等优点，因而可用于基层医疗单位。

Samples of serum and saliva in 70cases with acute hepatitis were examined for AntiHAV-IgM by ELISA.The results showed that 54 cases suffered from hepatitis A,19 of them were HAV and HBV coinfection.The positive results of Anti-HAV-IgM in serum and saliva were consistent in 50 cases(92.6%). In the control group of 81 cases, no one was positive for detection of Anti-HAV-IgM in serumandsaliva.Of 151 cases,thepositive and negative results were consistent in 147 cases(97. 4).Anti-HAV-IgM titers in saliva(ranged from 10-1 to 10-3) were lower than that in serum( ranged from 10-3 to 10-6).The lower titers did not affect the results of ELISA.This paper showed that posotive Anti-HAV-IgM in saliva could be as a mark of early diagnosis of hepatitis A.Because the saliva sample has many advantages, such as easy collecting,easy operating and easydetermination of test results, this method could be used in the basic medical units.