摘要
利用-20℃冰箱保存2年以上,OCT包理的冰冻组织标本提取DNA。结果表明,从清除掉OCT的组织中提取DNA与从新鲜手术标本中提取DNA效果基本一致,进而证实OCT对组织DNA无不良影响。可利用旧有材料进行回顾性研究,尚可对同一病例进行组织病理学及分子生物学对比研究。
DNAs from tissues embedded in the OCT,stored at -2O℃ more than 2
years and used once in the immunohistology research were extracted.The electrophoresis results
showed that the DNA bands were very clear and there was no harmful effect of OCT to
DNAs.Therefor,the authors think that the new method introduced here would be useful for
researchers because used samples can be re-used by this way and compared study can be done
using same samples,such as both histopathological and biological study.
出处
《中国医科大学学报》
CAS
CSCD
1994年第5期434-435,共2页
Journal of China Medical University