摘要
从鲤鱼内脏中提取的磷酸碱性酶(AKP)溶液, 经过正丁醇过夜脱脂、氯仿-95%乙醇去血红蛋白、丙酮沉淀、DEAE-琼脂糖层析柱、SephacrylS-200凝胶过滤,获得电泳纯磷酸碱性酶,最后纯化倍数为1 705. 90,活性回收率为7. 81。纯化AKP酶经聚丙稀酰胺凝胶电泳只呈现一条区带。
The alkaline phosphatase solution extracted from the internal organs of Cyprinus Carpio has been isolated and purified by butanol extraction ,hemoglobin dissolution withchloroform-95% ether , acetone precipitation,DEAE-sepharose column chromatography and Sephacryl S-200 gel filtration.The result is as following:purified times is 1705.90 and activity recovery rate is 7.81.The purified enzyme moved as a single electrophoretic band.
出处
《衡水学院学报》
2005年第1期13-14,共2页
Journal of Hengshui University
关键词
鲤鱼酸碱性酶
分离
纯化
Cyprinus Carpio
alkaline phosphatases
isolation,purification
