红细胞内超氧化物歧化酶活性测定及在小儿肺炎临床应用的意义

The Determination of Erythrocyte Superoxide Dismutase Activity and Its Clinical Signlficance in Child Pneumonia

作者就邻苯三酚自氧化法测定红细胞内ＳＯＤ活性的试验条件及样品制备进行了探讨。结果：邻苯三酚自氧化初速率４ｍｉｎ内呈直线性，且随其浓度及反应液ｐＨ的增加而增加；ＲＢＣ洗涤溶血后４℃放置，４８ｂ内酶活性不改变，ＳＯＤ提取液４℃放置２６天酶活性仍稳定，２０例健康小儿、２０例肺炎合并单纯心衰及１６例肺炎合并多系统器官功能衰竭患儿红细胞内ＳＯＤ活性的测定结果依次呈有意义的降低。提示：ＳＯＤ测定可做为某些疾病严重程度及预后判断的标，且可做为外源性抗氧化剂用于临床治疗。

The conditions for the determination of erythrocyte superoxide dismutase (SOD)activity by pyro-gallol autoxidation and for the sample preparation were studied. The results showed that the initial rateof pyrogallol autoxidation was linear during the first 4 mintes,the rate increased with the increase ofpyrogallol conce ntration and the pH in reaction fluid;The SOD activity in erythrocyte hemolysate wasstable after 48 hours at 4℃,and SOD activitv in extract was also stable after 26 days at 4℃,SoD ac-tivity in RBcs was investigated for 20 helthy children,20 pneumonia patients with simple heart failureand 16 pneumonia patients with multiple system organ failure. The results showed a significant decreasein order of group,indecating that the SOD activity assay can be used as an index of somed isease severi-ty and prognosis assessment.