转Bcl和Rip基因小麦的鉴定和遗传分析

Identification and Genetic Analysis of Transgenic Wheats with Bcl and Rip Genes

对转Bcl、Rip基因小麦的T1、T2和T3代植株进行了分子检测和抗病性鉴定,并根据检测结果对其遗传行为做了探讨与分析。T1代检测结果表明,选择标记基因nptⅡ的分离符合孟德尔遗传规律;功能基因Rip的分离比2.62∶1,符合孟德尔分离规律;功能基因Bcl的分离比为4.50∶1,偏离了孟德尔遗传规律。T1代植株Southernblot分析结果表明,功能基因Bcl和Rip已经稳定整合到小麦基因组上,多数植株为单拷贝整合。T2代纯合稳定植株Southernblot分析结果表明,功能基因Bcl和Rip在小麦染色体上多为双拷贝整合。白粉病和全蚀病鉴定结果表明,功能基因对白粉病和全蚀病均有一定的抗性。农艺性状调查结果表明,除了株高外转基因植株其它农艺性状与受体品种一致。

Detection at molecular level and anti-disease identification of the T1, T2 and T3 transgenic wheats with Bcl and Rip genes indicated that segregation ratios of nptⅡ selective gene and functional gene Rip were consistent with Mendel’s law,but functional gene Bcl was not in T1 generation. Southern blot showed that the alien genes had integrated into wheat genome and could be inherited to the following generations. The results also showed that there was just one copy of the alien genes in most T1 plants and most T2 homologous lines had two copies. After artificial inoculation of powdery mildew and wheat take-all, the transgenic plants demonstrated some resistance to them. And analysis of several agronomic traits indicated that transgenic plants had little difference in tiller, main spike length, spikelet number of main spike except plant height.