雷公藤单体T_4、T_7、T_（15）及雷公藤内酯醇对大鼠精子核蛋白组型的影响

The Effect of Tripterygium wilfordii Monomers T_4,T_7,T_(15)and Triptolide on Rat Nuclear Protein

大鼠喂服雷公藤单体Ｔ_４、Ｔ_７、Ｔ_（１５）及雷公藤内酯醇（甲素）７周后，取附睾精子，用超声法分离精子核，从核提取总碱性蛋白（ＴＮＢＰ）。经电泳扫描分析，发现精子ＴＮＢＰ含量下降，总组蛋白（ＴＨ）／精核蛋白（ｐｒｏｔａｒｍｉｎｅ）比值升高，精核蛋白含量下降，提示以上雷公藤单体和甲素使精子细胞核蛋白组型转换受阻是导致大鼠不育的重要原因之一。

Rat epididymal sperms were collected after 7 weeks of treatment with Tripterygium wilfordii monomers T_4,T_7,T_(15) and triptolide. Total nuclear basic protein(TNBP) were extracted from sperm nuclei isolated by sonication. The relative proportions of histones and protamine were determined by scanning microdensitometry following electrophoresis of TNBP in polyacrylamide gels. It was found that the content of TNBP was reduced while the total histone/protamine ratios were increased following treatment, indicating a marked decrease of protamine levels as compared with the control group. These results suggest that the interruption of nuclear protein transition of spermatids induced by T_4,T_7,T_(15) and triptolide might lead to infertility.